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利用微流体装置进行机械刺激和高分辨率成像的研究C.线虫

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

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利用微流体装置进行机械刺激和高分辨率成像的研究C.线虫

DOI:

10.3791/56530-v

•

10:39 min

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February 19, 2018

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Holger Fehlauer*¹, Adam L. Nekimken*^1,2, Anna A. Kim², Beth L. Pruitt^2,3, Miriam B. Goodman², Michael Krieg

¹Department of Molecular and Cellular Physiology,Stanford University, ²Department of Mechanical Engineering,Stanford University, ³Department of Bioengineering,Stanford University, ⁴Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

Chapters

00:05Title
00:52Preparation of the Microscope
02:02Preparing the Microfluidic Chip
03:45Loading the Test Subject
06:02Stimulation and Removal of the Test Subject
08:13Results: Touch Receptor Neuron (TRN) Stimuli
09:42Conclusion

Summary

Automatic Translation

美国匹茨堡研究的新工具需要了解机械应力是如何激活生化通路并引起生物学反应的。在这里, 我们展示了一种新的方法, 选择性机械刺激固定化动物与微流控器陷阱, 允许高分辨率成像的细胞反应。

Tags

Microfluidics Mechanical Stimulation High-resolution Imaging C. Elegans Mechanobiology Sensory Biology Pressure Actuators GCaMP RFP Fluorescence Microscopy M9 Buffer Peristaltic Pump PDMS

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