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Journal
/
Immunology and Infection
/
サルモネラ
の分離-大食細胞のファゴゾームを含む
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of
Salmonella typhimurium
-containing Phagosomes from Macrophages
Please note that all translations are automatically generated.
Click here for the English version.
サルモネラ
の分離-大食細胞のファゴゾームを含む
DOI:
10.3791/56514-v
•
10:33 min
•
October 25, 2017
•
Saray Gutiérrez
2
,
Martina Wolke
,
Georg Plum
,
Nirmal Robinson
2
1
Institute for Medical Microbiology, Immunology and Hygiene
,
University of Cologne
,
2
Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD)
,
University of Cologne
Chapters
00:05
Title
01:03
Salmonella typhimurium
Culture
02:17
Coating of Bacteria with NHS-biotin/Streptavidin-conjugated Magnetic Beads
04:38
Bone Marrow-derived Macrophages (BMDM) Infection
06:18
Isolation of
S. typhimurium
-containing Phagosomes
08:21
Results: Successful Biotinylation of Bacterial and Isolation of Bacterial-containing Phagosomes
09:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
簡単で迅速な
サルモネラ
の分離法をご紹介-ビオチンとストレプトアビジン菌をコーティングすることによりマクロファージからファゴゾームを含みます。
Tags
Salmonella Typhimurium
Phagosomes
Macrophages
Isolation
Immunology
Antigen Processing
Intracellular Pathogens
Biotin
Streptavidin-conjugated Magnetic Beads
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