Journal
/
/
サンプル準備および透過電子顕微鏡によるエクソソームのイメージ投射
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
DOI:

11:15 min

January 04, 2018

,

Chapters

  • 00:05Title
  • 01:14Exosome Block Preparation
  • 03:38Sectioning, Staining, and Imaging of the Exosome
  • 04:27Immuno-staining of Sections and Imaging
  • 07:24Whole Mount for Immunostaining
  • 09:10Results: Immunostained Exosome Sections Provide Insight to Their Protein Makeup
  • 09:59Conclusion

Summary

Automatic Translation

このプロトコルでは、否定的な汚損、詳細な構造と免疫金エクソソームの特定の蛋白質の位置を判断するための極薄断面透過型電子顕微鏡に必要な様々 なテクニックについて説明します。

Related Videos

Read Article