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封闭系统中脑切片长期培养中精确定位和重复间歇成像的改进辊筒法
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Neuroscience
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JoVE Journal Neuroscience
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System
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封闭系统中脑切片长期培养中精确定位和重复间歇成像的改进辊筒法

DOI:
10.3791/56436-v

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09:52 min

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December 28, 2017

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, , , , , , , ,
1Department of Biochemistry and Molecular Biology and Molecular, Cellular and Integrated Neuroscience Program,Colorado State University, 2IBMC-Instituto de Biologia Molecular e Celular, i3S-Instituto de Investigaçãoe Inovação em Saúde, ICBAS,Universidade do Porto, 3Denali Therapeutics

Chapters

  • 00:05Title
  • 01:28Preparation of Roller Tubes and Coverslips
  • 02:51Plating Slices
  • 05:44Slice Imaging
  • 07:28Results: Repetitive Intermittent Imaging of Brain Slices During Long-term Culture
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了一种改进的滚子管法培养和间歇高分辨率成像啮齿动物脑切片多周, 精确重新定位光刻片。神经元的生存能力和切片形态学保持良好。提供了这种完全封闭的系统使用病毒的细胞类型的特定表达的应用。

Tags

Brain Slice CultureLong-term CultureRepetitive ImagingFluorescence ImagingEnclosed SystemRoller Tube MethodPhoto Etched CoverslipsViral VectorsNeurological DisordersSynapse AlterationRodent Models

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