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重叠肽库在蛋白质 Qa-1 表位中的映射
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Immunology and Infection
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JoVE Journal Immunology and Infection
Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
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重叠肽库在蛋白质 Qa-1 表位中的映射

DOI:
10.3791/56401-v

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08:04 min

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December 20, 2017

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, , , ,
1Department of Medicine, Division of Regenerative Medicine,Loma Linda University

Chapters

  • 00:05Title
  • 00:56Overlapping Peptide (OLP) Library Generation and T Cell Priming with OLP Pool-pulsed Dendritic Cells (DCs) Protocol
  • 03:43Re-stimulation of OLP Pool-primed CD8+ T Cells Using Macrophages Pulsed with the OLP Pool
  • 06:19Results: Identification of a 9-mer Qa-1 Epitope in Myelin Oligodendrocyte Glycoprotein (MOG)
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

Qa-1 (HLA) 属于一组经典主要组织相容性复杂的1b 分子。免疫 Qa-1-binding 表位已表明, 以加强组织特异性免疫调节和改善一些自身免疫性疾病。在此, 我们描述一个重叠的肽库策略, 以确定 Qa-1 表位的蛋白质。

Tags

Overlapping Peptide LibraryQa-1 EpitopesImmune RegulationImmunotherapyMultiple SclerosisHLA-EDendritic CellsCD8+ T CellsInterleukin 2Interleukin 7

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