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基于芯片的三维细胞培养,在灌注微生物反应器
JoVE Journal
Biology
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JoVE Journal Biology
Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
DOI:

12:39 min

May 21, 2008

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Chapters

  • 00:08Introduction
  • 00:27CellChip preparation: Hydrophilization, coating, incubation
  • 03:41Bioreactor Setup
  • 07:43Incubation Setup
  • 08:58Analysis of organotypic functions

Summary

Automatic Translation

我们描述了一个在微型生物反应器培养细胞的三维芯片为基础的平台。一个芯片上可以容纳10百万。无菌,封闭的循环回路中流体流动,氧张力等方面的准确界定条件下,可以培养细胞。

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