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Journal
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Immunology and Infection
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实时颤振诱发的粪便中 CWD 朊的转化检测
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
Please note that all translations are automatically generated.
Click here for the English version.
实时颤振诱发的粪便中 CWD 朊的转化检测
DOI:
10.3791/56373-v
•
09:50 min
•
September 29, 2017
•
Yo Ching Cheng*
1
,
Samia Hannaoui*
1
,
Theodore Ralph John
,
Sandor Dudas
,
Stefanie Czub
,
Sabine Gilch
1
Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine
,
University of Calgary
,
2
Dept. of Molecular Biology
,
University of Wyoming
,
3
Canadian Food Inspection Agency
,
Lethbridge Laboratories
Chapters
00:05
Title
00:42
Preparation of Cervid Feces Etracts
01:48
Concentration of PrP
03:33
Real-time Quaking-induced Conversion Assay
07:28
Results: Detection of CWD Prions in Fecal Material
08:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出一个协议来描述一个简单, 快速和有效的朊病毒放大技术, real-time 颤抖诱导转换 (RT-QuIC) 方法。
Tags
CWD Prions
Fecal Material
Real-time Quaking-induced Conversion Assay
Cervid
Chronic Wasting Disease
Sensitive Detection
High Throughput
Sodium Phosphotungstic Acid Precipitation
RT-QuIC Assay
Thioflavin T
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