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Cancer Research
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细胞侵袭和迁移过程的评价: 视频 Microscope-based 刮伤法与博伊登室法的比较
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay
Please note that all translations are automatically generated.
Click here for the English version.
细胞侵袭和迁移过程的评价: 视频 Microscope-based 刮伤法与博伊登室法的比较
DOI:
10.3791/56337-v
•
11:20 min
•
November 17, 2017
•
Jean-Baptiste Guy*
1,2
,
Sophie Espenel*
1,2
,
Alexis Vallard
2
,
Priscillia Battiston-Montagne
,
Anne-Sophie Wozny
3
,
Dominique Ardail
3
,
Gersende Alphonse
3
,
Chloé Rancoule
2
,
Claire Rodriguez-Lafrasse
3
,
Nicolas Magne
2
1
UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud
,
Université Lyon 1
,
2
Département de Radiothérapie
,
Institut de Cancérologie de la Loire – Lucien Neuwirth
,
3
Hospices Civils de Lyon
,
Centre Hospitalier Lyon-Sud
Chapters
00:05
Title
00:58
Boyden Chamber Assay
03:58
Scratch Wound Assay for Cell Migration
07:09
Scratch Wound Assay for Cell Invasion
09:35
Results: A Video Microscope-based Scratch Wound Assay is More Advantageous than the Boyden Assay
11:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究报告了两种不同的细胞浸润和迁移分析方法: 博伊登室法和
体外
视频 microscope-based 伤口愈合试验。对这两种实验的协议进行了描述, 并对它们的优缺点进行了比较。
Tags
Cell Invasion
Cell Migration
Scratch Wound Assay
Boyden Chamber Assay
Video Microscopy
SQ20B Cells
Cell Culture
Cell Starvation
Chemoattractant
Invasion Assay
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