Journal
/
Cancer Research
/
基于 rna 的液体活检: 血浆 miRNA 特征分类器 (MSC) 在肺癌筛查中的经验
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening
Please note that all translations are automatically generated. Click here for the English version.

基于 rna 的液体活检: 血浆 miRNA 特征分类器 (MSC) 在肺癌筛查中的经验

DOI:
10.3791/56326-v

•

08:14 min

•

October 26, 2017

•

, , , , , , , ,
1Department of Experimental Oncology and Molecular Medicine,Fondazione IRCCS Istituto Nazionale dei Tumori, 2Unit of Thoracic Surgery,Fondazione IRCCS Istituto Nazionale dei Tumori

Chapters

  • 00:05Title
  • 01:12Plasma Sample Collection and RNA Extraction
  • 03:20Taq-based Reverse Transcription and Pre-amplification
  • 04:34RT-qPCR Reaction
  • 05:46Results: Quality Control of Plasma Samples and RT-qPCR Performance Evaluation
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了在 BioMILD 筛选试验中采用的详细的协议, 以执行循环 rna 签名分类器测试早期肺癌的检测。

Tags

Liquid BiopsyPlasma MiRNALung Cancer ScreeningMicroRNA SignatureEarly DetectionTumor MicroenvironmentRNA IsolationQPCRMolecular Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

利用功能基因组筛选识别在肿瘤细胞球体培养潜在的新药物靶标

流动 Cytometry-based 药物筛选系统对促进细胞分化胶质母细胞瘤小分子的鉴定

应用肺转移法研究骨肉瘤转移性肺定植的实用思考

肺定植法在肺部组织循环肿瘤细胞可视化中的建立

pT3N0M0 胃癌组织石蜡嵌入幻灯片上的弹性染色

利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞

肿瘤病理学鼠肺的灌注和膨胀

肿瘤细胞外侧尾静脉注射后肺转移的病理分析

通过颅内注射和磁共振成像对大脑转移进行建模

癌症患者血浆样本中无细胞DNA检测

Read Article