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Immunology and Infection
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激光内在人肺组织中的新型细菌特异探针的光学筛选
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Optical Screening of Novel Bacteria-specific Probes on
Ex Vivo
Human Lung Tissue by Confocal Laser Endomicroscopy
Please note that all translations are automatically generated.
Click here for the English version.
激光内在人肺组织中的新型细菌特异探针的光学筛选
DOI:
10.3791/56284-v
•
09:24 min
•
November 29, 2017
•
Bethany Mills
,
Ahsan R. Akram
,
Emma Scholefield
,
Mark Bradley
,
Kevin Dhaliwal
1
EPSRC Proteus Hub, MRC Centre of Inflammation Research, Queen’s Medical Research Institute
,
University of Edinburgh
,
2
School of Chemistry, EaStChem
,
University of Edinburgh
Chapters
00:05
Title
00:44
Preparation of
Ex Vivo
Human Lung Tissue
01:53
Setup of the Confocal Laser Endomicroscopy (CLE) Device
03:55
Data Collection with CLE
05:59
Data Analysis
07:41
Results: FCFM Imaging of Labeling Bacteria with Human Lung Tissue
08:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项技术描述了一个有效的筛选过程, 以评估细菌特异性光学成像剂在
前体内
人肺组织, 通过纤维共聚焦荧光显微镜快速鉴定小分子化学具有可翻译潜力的候选者。
Tags
Optical Imaging
Confocal Laser Endomicroscopy
Bacteria-specific Probes
Molecular Imaging
Imaging Probes
Calcein AM-labeled Bacteria
Fibered Confocal Fluorescence Microscopy
Laser Scanning Unit
FCFM Imaging Fiber Calibration
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