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Journal
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Immunology and Infection
/
在体内
应用免疫模型检测抗原特异性 T 细胞细胞功能的试验
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
In Vivo
Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
Please note that all translations are automatically generated.
Click here for the English version.
在体内
应用免疫模型检测抗原特异性 T 细胞细胞功能的试验
DOI:
10.3791/56255-v
•
07:05 min
•
November 28, 2017
•
Cara L. Haymaker*
1
,
Yared Hailemichael*
1
,
Yi Yang
3
,
Roza Nurieva
1
Department of Melanoma Medical Oncology
,
University of Texas MD Anderson Cancer Center
,
2
Department of Immunology
,
University of Texas MD Anderson Cancer Center
,
3
Department of Radiation Oncology
,
The Second Hospital of Jilin University
Chapters
00:05
Title
01:00
Administration of the Immunostimulatory Cocktail Covax
01:57
Isolation of Target Splenocytes for Labeling with CFSE and Peptide Pulsing of Target Splenocytes
02:55
Preparation of CFSE and Labeling of Target Splenocytes
04:52
Results: Detection of Antigen-specific T-cell Cytolytic Function
06:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目标是允许检测在小鼠模型中的靶细胞的
体内
抗原特异性杀伤。
Tags
In Vivo Assay
Antigen-specific T-cell Cytolytic Function
Vaccination Model
Antigen-specific Killing
Cytolytic Potential
Peptide-pulsing
CSFE Labeling
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