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1Department of Clinical Research,University of Bern, 2Graduate School for Cellular and Biomedical Sciences,University of Bern, 3Immunology Research Centre,St. Vincent’s Hospital Melbourne, 4Institute of Pathology,University of Bern, 5Institute of Molecular Animal Breeding and Biotechnology,Ludwig-Maximilian University
Chapters
- 00:05Title
- 00:48Coating Microcarrier Beads
- 01:39Collecting Cells
- 02:26Seeding Cells into the Stirrer Flask
- 03:45Experimental Procedure
- 04:32Incubation with Non-anticoagulated Blood
- 05:46Results: Clotting
- 06:28Conclusion
Nous présentons un modèle in vitro qui permet l’étude et l’analyse de la coagulation dans le sang entier, de non anticoagulé. Anticoagulation dans le système dépend de l’effet anticoagulant naturel des cellules endothéliales saines et activation des cellules endothéliales se traduira par la coagulation.
Tags
3D Cell CultureEndothelial CellsAnticoagulant PropertiesAnti-inflammatory PropertiesWhole BloodNon-anticoagulated BloodCardiovascular ResearchTransportation ResearchSurgery ResearchAnti-immunityEndothelial Cell ActivationMicrocarrier BeadsCoagulationPhosphate Buffered Saline (PBS)Dulbecco’s Modified Eagle’s Medium (DMEM)Medium 199TrypsinEDTACell Culture MediaMagnetic Stirrer Flask
