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利用胶囊对生物达标中病毒样品的阴性染色
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment
DOI:

08:22 min

July 19, 2017

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Chapters

  • 00:05Title
  • 01:08Experiment Preparation in a BSL-2 Environment Prior to Working with the Virus Samples (Biocontainment Procedures were Filmed in BSL-4 Training Lab)
  • 01:45The Capsule Method for Negative Staining in Biocontainment Using Aqueous Glutaraldehyde and 1% Osmium Tetroxide Vapor Inactivation
  • 04:24The Capsule Method for Inactivation in Biocontainment 2% Glutaraldehyde Followed by Negative Staining in BSL-2 Laboratory
  • 05:50Results: Analyzing the Capsule Method of Negative Staining Viral Samples
  • 07:14Conclusion

Summary

Automatic Translation

该协议为负染色病毒样本提供了指导,可以很容易地用于BSL-2,-3或-4实验室。它包括使用创新的加工胶囊,其保护透射电子显微镜网格并且在生物达到的更湍流环境中为使用者提供更容易的处理。

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