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用可寻址滴微阵列计算单细胞中的蛋白质
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JoVE Journal
Cancer Research
Counting Proteins in Single Cells with Addressable Droplet Microarrays
Please note that all translations are automatically generated.
Click here for the English version.
用可寻址滴微阵列计算单细胞中的蛋白质
DOI:
10.3791/56110-v
•
12:25 min
•
July 06, 2018
•
Stelios Chatzimichail*
1
,
Pashiini Supramaniam*
1
,
Oscar Ces
,
Ali Salehi-Reyhani
1
Institute of Chemical Biology, Department of Chemistry
,
Imperial College London
Chapters
00:05
Title
00:55
Prepare Apparatus for Dispensing Addressable Droplets
03:26
Form Addressable Droplets and Load with Single Cells
07:42
Data Analysis
09:39
Results: Single Cell Data
11:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了可寻址的液滴微阵列 (ADMs), 一种能够确定单细胞中绝对蛋白质丰度的液滴数组方法。我们展示了 ADMs 的能力来表征肿瘤抑制蛋白 p53 在人类癌细胞系中表达的异质性。
Tags
Single Cell
Protein Copy Number
Addressable Droplet Microarray
Heterogeneity
Central Dogma
Gene Expression
Fused Silica Tubing
Concentric Nozzle
PTFE Tubing
FEP Tubing
Hamilton Syringe
Cyanoacrylate Glue
PBSA Blocking Solution
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