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使用凝胶内杂交来定量端粒长度的修饰末端限制片段分析
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JoVE Journal
Cancer Research
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
Please note that all translations are automatically generated.
Click here for the English version.
使用凝胶内杂交来定量端粒长度的修饰末端限制片段分析
DOI:
10.3791/56001-v
•
11:29 min
•
July 10, 2017
•
Frank J. Jenkins
2
,
Charles M. Kerr
,
Elise Fouquerel
3
,
Dana H. Bovbjerg
4
,
Patricia L. Opresko
3
1
Departments of Pathology and Infectious Diseases and Microbiology
,
University of Pittsburgh
,
2
University of Pittsburgh Cancer Institute
,
3
Department of Environmental and Occupational Health
,
University of Pittsburgh
,
4
Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences
,
University of Pittsburgh
Chapters
00:05
Title
00:46
Genomic DNA Digestion
01:39
Genomic DNA Gel Electrophoresis
02:56
Gel Fluorescent Imaging and Hybridization
05:17
Hybridization with Telomere Probe
07:36
Gel Washing, Phospher Screen Exposure, and Radio Imaging
09:08
Results: Telomere Length is Quantified by a Modefied Terminal Restriction Fragment Analysis
10:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
在本文中,讨论了使用修饰的末端限制性片段分析量化端粒长度的详细方案,其提供了端粒长度的快速和有效的直接测量。该技术可以应用于DNA的各种细胞来源以量化端粒长度。
Tags
Telomere Length
Modified Terminal Restriction Fragment Analysis
In-gel Hybridization
Eukaryotic Cells
Cell Biology
Epidemiology
Infectious Diseases
DNA Extraction
DNA Digestion
Agarose Gel Electrophoresis
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