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使用凝胶内杂交来定量端粒长度的修饰末端限制片段分析
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
DOI:

11:29 min

July 10, 2017

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Chapters

  • 00:05Title
  • 00:46Genomic DNA Digestion
  • 01:39Genomic DNA Gel Electrophoresis
  • 02:56Gel Fluorescent Imaging and Hybridization
  • 05:17Hybridization with Telomere Probe
  • 07:36Gel Washing, Phospher Screen Exposure, and Radio Imaging
  • 09:08Results: Telomere Length is Quantified by a Modefied Terminal Restriction Fragment Analysis
  • 10:28Conclusion

Summary

Automatic Translation

在本文中,讨论了使用修饰的末端限制性片段分析量化端粒长度的详细方案,其提供了端粒长度的快速和有效的直接测量。该技术可以应用于DNA的各种细胞来源以量化端粒长度。

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