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Immunology and Infection
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使用madSTORM进行高分辨率,超分辨率的T细胞成像
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
Please note that all translations are automatically generated.
Click here for the English version.
使用madSTORM进行高分辨率,超分辨率的T细胞成像
DOI:
10.3791/55997-v
•
08:43 min
•
June 24, 2017
•
Jason Yi
,
Asit Manna
,
Valarie A. Barr
,
Jennifer Hong
,
Keir C. Neuman
,
Lawrence E. Samelson
1
Laboratory of Cellular & Molecular Biology, National Cancer Institute
,
National Institutes of Health
,
2
Laboratory of Single Molecule Biophysics, National Heart, Lung, and Blood, Institute
,
National Institutes of Health
Chapters
00:05
Title
00:36
Preparation of Fluorescent Nano-diamond-coated Coverslips
02:49
Activation of Jurkat T Cells
04:15
Imaging Activated Jurkat T Cells Using madSTORM
07:00
Results: Multiplexed Super-resolution Image of Proteins in an Activated T Cell
07:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们展示了使用荧光标记抗体的顺序结合和洗脱以单分子精确度在异质纳米结构内成像多个分子的方法。
Tags
T Cells
MadSTORM
Super-resolution Imaging
Multiplexed Imaging
Single Molecule Localization Microscopy
Alexa 647
Poly-L-lysine
Fluorescent Nanodiamonds
Anti-CD3 Antibody
Jurkat T Cells
HBS Solution
Article
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