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Journal
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Immunology and Infection
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基于磁珠复合法的撕裂细胞因子分析
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
Please note that all translations are automatically generated.
Click here for the English version.
基于磁珠复合法的撕裂细胞因子分析
DOI:
10.3791/55993-v
•
09:22 min
•
October 13, 2017
•
Praveen Kumar Balne
2
,
Veonice Bijin AU
,
Louis Tong
,
Arkasubhra Ghosh
,
Mukesh Agrawal
,
John Connolly
,
Rupesh Agrawal
1
National Healthcare Group Eye Institute
,
Tan Tock Seng Hospital
,
2
Singapore Eye Research Institute (SERI)
,
3
Institute of Molecular and Cell Biology
,
A*STAR
,
4
GROW Research Laboratory
,
Narayana Nethralaya Foundation
,
5
Vimta Labs Limited
Chapters
00:05
Title
00:42
Elution of Tear Sample from Tear Flow Strip
01:26
Cytokine Profiling by Bead Based Multiplex Assay
04:43
Reading the Assay Plate and Data Analysis
06:56
Results: Tear Cytokine Profiles from Four Male Subjects
08:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
泪膜细胞因子的分析有助于研究各种眼部疾病。基于微珠的复合检测方法简单、灵敏, 能够对小体积样品中的多个目标进行测试。在这里, 我们描述了一个协议的撕裂膜细胞因子分析一个使用珠基复用法。
Tags
Tear Cytokine Profile
Bead-based Multiplex Assay
Schirmer Strip
Ophthalmology
Cytokines
Ocular Diseases
Analytes
Tear Sample
Assay Buffer
Centrifugation
Cytokine Profiling
Multiplex Assay
Cytokine Standards
Assay Worksheet
Magnetic Plate Washer
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