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通过RNA定位蝗虫天线中的气味受体基因<em>原位</em>杂交

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

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通过RNA定位蝗虫天线中的气味受体基因<em>原位</em>杂交

DOI:

10.3791/55924-v

•

09:30 min

•

July 13, 2017

•

Xiao Xu, Yinwei You², Long Zhang

¹Department of Entomology,China Agricultural University, ²Bio-tech Research Center,Shandong Academy of Agricultural Sciences

Chapters

00:05Title
00:55Preparation of Cryostat Sections
01:59Fixing Sections and Hybridization
03:30Washing, Blocking, and Immunohistochemistry
05:10Staining
07:02Results: Neuronal Identity of Antennal Cells Expressing LmigORs by Chromogenic and TSA Detections
08:27Conclusion

Summary

Automatic Translation

本文描述了一种详细和高效的RNA 原位杂交方案，特别是对于低水平表达的气味受体（OR）基因以及其他基因，在使用地高辛（DIG）标记或生物素标记的探针的昆虫天线中。

Tags

Odorant Receptor Genes RNA In Situ Hybridization Locust Antennae Digoxigenin Labeled Probes Biotin Labeled Probes Freezing Microtome Cryostat Sections 4% PFA 0.2 M HCl 1X PBS 1% Triton X-100 Formamide Antisense Probe Sense Probe 0.1X SSC 1X TBS 1% Blocking Reagent Chromogenic Detection

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