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转移因子的鉴定和表征转移到新的RIP 标签;tva小鼠模型
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Cancer Research
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JoVE Journal Cancer Research
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
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转移因子的鉴定和表征转移到新的RIP 标签;tva小鼠模型

DOI:
10.3791/55890-v

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09:03 min

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October 16, 2017

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, ,
1Department of Pathology and Laboratory Medicine,Weill Cornell Medicine

Chapters

  • 00:05Title
  • 00:46Collection and Concentration of Viruses Expressing Metastatic Factor
  • 02:26Viral Titer Determination
  • 04:37Intracardiac Injection
  • 07:24Results: Detection of Metastatic Pancreatic β-cells by Immunostaining
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

本文提出了一种利用 RIP 标记来演示一种新的体细胞基因转移系统的协议;tva小鼠模型研究基因在转移中的作用。禽病毒的 intracardiacally, 以确保基因转移到前, 无创性病变的胰β细胞在成年小鼠。

Tags

Metastatic FactorsGene TransferRIP-Tag; RIP-tva Mouse ModelPancreatic Beta Cell Precursor LesionsVirus Producing DF1 CellsViral SupernatantUltracentrifugationViral TiterSerial DilutionDF1 Cells

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