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小鼠小脑颗粒神经元神经元死亡和变性的模型研究

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

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小鼠小脑颗粒神经元神经元死亡和变性的模型研究

DOI:

10.3791/55871-v

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10:36 min

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November 06, 2017

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Matthew Laaper², Takrima Haque, Ruth S. Slack, Arezu Jahani-Asl^2,4

¹Lady Davis Institute for Medical Research,Jewish General Hospital, ²Integrated Program in Neuroscience,McGill University, ³Department of Cellular and Molecular Medicine,University of Ottawa, ⁴Department of Oncology, Faculty of Medicine,McGill University

Chapters

00:05Title
01:11Brain Dissection and Isolation of the Cerebellum
03:11Mouse Cerebellar Granule Neuron Isolation and Culturing
06:38Modeling Neuronal Injury
08:09Results: Representative Images of Cerebellar Granule Neurons Infected with Different MOIs
09:36Conclusion

Summary

Automatic Translation

本协议描述了一种简单的方法, 分离和培养的小鼠脑颗粒神经元 (CGNs) 从6-7 天的老幼崽, 有效的转导 CGNs 的损失和增益的功能研究, 并建模 NMDA 诱导神经元兴奋,低诱导的细胞死亡, DNA 损伤, 和氧化应激使用相同的培养模式。

Tags

Cerebellar Granule Neurons Neuronal Death Neurodegeneration Primary Cell Culture Excitotoxicity Oxidative Stress DNA Damage Developmental Events Neuronal Activity Morphogenesis Growth Factors Mouse Brain Dissection Cerebellum Isolation Meningeal Removal

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