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开发HiPSC衍生的无血清胚胎体用于使用生理相关测定的3-D干细胞培养物的询问
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays
DOI:

10:43 min

July 20, 2017

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Chapters

  • 00:05Title
  • 01:04Generation of Neural Progenitor Cell
  • 04:02Serum-free Embryoid Body (SFEB) Induction
  • 06:01Recording Neuronal Activity in SFEBs using MEAs
  • 08:09Results: SFEB Characteristics
  • 09:51Conclusion

Summary

Automatic Translation

在这里,我们报告了一种由称为无血清胚状体(SFEB)的人诱导多能干细胞(hiPSCs)开发三维(3-D)系统的方案。这种3-D模型可以像器官切片培养物一样用于模拟人类皮质发育和生理学询问发展中的神经回路。

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