JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
葡萄糖摄取测量和对胰岛素刺激的反应<em>体外</em>培养的人类初级肌管
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Glucose Uptake Measurement and Response to Insulin Stimulation in
In Vitro
Cultured Human Primary Myotubes
Please note that all translations are automatically generated.
Click here for the English version.
葡萄糖摄取测量和对胰岛素刺激的反应<em>体外</em>培养的人类初级肌管
DOI:
10.3791/55743-v
•
08:03 min
•
June 25, 2017
•
Stephanie Chanon
,
Christine Durand
,
Aurelie Vieille-Marchiset
,
Maud Robert
,
Charna Dibner
,
Chantal Simon
,
Etienne Lefai
1
CarMeN Laboratory, INSERM U1060, INRA 1397
,
University of Lyon
,
2
Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon
,
Lyon 1 University
,
3
Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine
,
University of Geneva
Chapters
00:05
Title
00:34
Cell Culture Media and Solution Preparation
02:45
Culture of Human Primary Muscle Cells
04:19
Insulin Stimulation and Glucose Uptake
05:30
Cell Lysis
06:06
Determination of Radiolabeled Glucose
06:52
Results: Effective Glucose Uptake Measurement and Analysis of Response to Insulin Stimulation
07:37
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这种方法中,人原代肌细胞
在体外培养
以获得分化的肌管,并测量葡萄糖摄取率。我们提供了一个详细的方案,使用放射性标记的[
3
H] 2-脱氧-D-葡萄糖来量化基础和胰岛素刺激状态的速率。
Tags
Glucose Uptake
Insulin Stimulation
Human Primary Myotubes
Muscle Metabolism
Insulin Sensitivity
Proliferation Medium
Differentiation Medium
2DG
Cytochalasin B
DMSO
Muscle Satellite Cells
Cell Culture
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在原代培养海马神经元的密集核心囊泡的实时成像
电离辐射的反应定量的γH2AX灶
注射胰岛素和血淋巴的提取来衡量成人胰岛素敏感性
果蝇</em
大鼠肝细胞的分离和小学文化
核糖体束缚新生多肽的分离<em>在体外</em>确定沿mRNA的翻译暂停网站
细胞器运输的培养<em>果蝇</em>细胞:S2细胞系和原代神经元。
细胞质DNA的检测途径的刺激<em>在体外</em>和<em>体内</em
测量水润谷胱甘肽诱导饲养响应
沉默<em> BRCA2</em>以确定新的BRCA2调控的生物学功能在人体细胞培养
间隙连接通讯:天然产物的生物标志物的功能,以评估毒物和毒素的不利影响,以及健康的益处
Read Article