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葡萄糖摄取测量和对胰岛素刺激的反应<em>体外</em>培养的人类初级肌管
JoVE Journal
Biology
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JoVE Journal Biology
Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
DOI:

08:03 min

June 25, 2017

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Chapters

  • 00:05Title
  • 00:34Cell Culture Media and Solution Preparation
  • 02:45Culture of Human Primary Muscle Cells
  • 04:19Insulin Stimulation and Glucose Uptake
  • 05:30Cell Lysis
  • 06:06Determination of Radiolabeled Glucose
  • 06:52Results: Effective Glucose Uptake Measurement and Analysis of Response to Insulin Stimulation
  • 07:37Conclusion

Summary

Automatic Translation

在这种方法中,人原代肌细胞在体外培养以获得分化的肌管,并测量葡萄糖摄取率。我们提供了一个详细的方案,使用放射性标记的[ 3 H] 2-脱氧-D-葡萄糖来量化基础和胰岛素刺激状态的速率。

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