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在侧群的分离的Myc诱导的T细胞急性淋巴细胞白血病斑马鱼
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish
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在侧群的分离的Myc诱导的T细胞急性淋巴细胞白血病斑马鱼

DOI:
10.3791/55711-v

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09:06 min

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May 04, 2017

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, , ,
1Department of Pediatrics, Section of Hematology-Oncology,The University of Chicago, 2Department of Biology and Biotechnology,Worcester Polytechnic Institute

Chapters

  • 00:05Title
  • 01:07Isolation of Fluorescently Labeled T-ALL Cells from Zebrafish
  • 04:01Identification of Side Population Cells in Zebrafish T-ALL
  • 06:53Results: Side Population Identification in T-ALL Cells from Zebrafish
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一种从myc诱导的T细胞急性淋巴细胞白血病（T-ALL）的斑马鱼模型中分离侧群体细胞的技术。这种侧群体测定是高度敏感的，描述为斑马鱼T-ALL，但它可能适用于其他恶性和非恶性斑马鱼细胞类型。

Tags

Side PopulationT-cell Acute Lymphoblastic LeukemiaZebrafishMyc-induced CancerCancer Stem CellsCell IsolationHeparinEuthanasiaFBS/PBSCell Collection

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