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Journal
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Biology
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活细胞成像血小板脱水和分泌流量
JoVE Journal
Biology
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JoVE Journal
Biology
Live-cell Imaging of Platelet Degranulation and Secretion Under Flow
Please note that all translations are automatically generated.
Click here for the English version.
活细胞成像血小板脱水和分泌流量
DOI:
10.3791/55658-v
•
11:42 min
•
July 10, 2017
•
Arjan D. Barendrecht*
1
,
Johan J. F. Verhoef*
2
,
Silvia Pignatelli
,
Gerard Pasterkamp
,
Harry F. G. Heijnen
,
Coen Maas
1
Department of Clinical Chemistry and Haematology
,
University Medical Center Utrecht
,
2
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences
,
Utrecht University
Chapters
00:05
Title
01:12
Cover Glass Preparation
02:25
Platelet Isolation and Washing
03:58
Flow Chamber Assembly
05:08
Pre-strained Platelets and Microscope Setup
07:36
Microscope Imaging of Platelets
09:31
Results: Platelet Degranulation and Secretion Under Flow
10:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项工作描述了基于荧光显微镜的方法研究血小板粘附,扩散和分泌的流动。这种多功能平台能够调查血小板功能,用于血栓形成和止血的机械研究。
Tags
Live-cell Imaging
Platelet Degranulation
Platelet Secretion
Flow
Platelet Biology
Platelet Isolation
Platelet Preactivation
Von Willebrand Factor
Fibrinogen
HEPES Tyrode Buffer
Platelet-rich Plasma
Citrated Whole Blood
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