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Journal
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Immunology and Infection
/
血液滴からの迅速な好中球走化性解析のためのオールオンチップ法
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Immunology and Infection
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Immunology and Infection
An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood
Please note that all translations are automatically generated.
Click here for the English version.
血液滴からの迅速な好中球走化性解析のためのオールオンチップ法
DOI:
10.3791/55615-v
•
07:21 min
•
June 23, 2017
•
Ke Yang*
1,2,3
,
Jiandong Wu*
3,4
,
Ling Zhu
,
Yong Liu
,
Michael Zhang
,
Francis Lin
4,6,7
1
Institute of Applied Technology, Hefei Institutes of Physical Science
,
Chinese Academy of Sciences
,
2
University of Science and Technology of China
,
3
Department of Physics and Astronomy
,
University of Manitoba
,
4
Department of Biosystems Engineering
,
University of Manitoba
,
5
Seven Oaks General Hospital
,
6
Department of Immunology
,
University of Manitoba
,
7
Department of Biological Sciences
,
University of Manitoba
Chapters
00:05
Title
00:46
Microfluidic Device Fabrication
03:06
Microfluidic Cell Migration Assay Preparation
03:52
On-chip Cell Isolation and Chemotaxis Assay
05:46
Results: All-on-chip Neutrophil Chemotaxis Response to fMLP Chemical Gradient
06:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
この記事では、全血からのチップ上の好中球単離と、単一のマイクロ流体チップ上の走化性試験とを統合することにより、迅速な好中球走化性アッセイを行う詳細な方法を提供する。
Tags
Neutrophil Chemotaxis
Microfluidic Device
Whole Blood
Neutrophil Separation
Cell Migration
Chemotaxis Assay
PDMS
SU8 Mold
Plasma Treatment
Fibronectin
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