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从成年大鼠心脏同时分离高质量心肌细胞,内皮细胞和成纤维细胞
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Biology
Simultaneous Isolation of High Quality Cardiomyocytes, Endothelial Cells, and Fibroblasts from an Adult Rat Heart
Please note that all translations are automatically generated.
Click here for the English version.
从成年大鼠心脏同时分离高质量心肌细胞,内皮细胞和成纤维细胞
DOI:
10.3791/55601-v
•
09:38 min
•
May 19, 2017
•
Dursun Gündüz
,
Christian W. Hamm
,
Muhammad Aslam
1
Internal Medicine, Cardiology and Angiology
,
University Hospital Giessen
Chapters
00:05
Title
00:55
Heart Langendorff Perfusion and Digestion
03:43
Cardiomyocyte Isolation
05:08
Endothelial Cell and Fibroblast Isolation
07:45
Results: Representative Cardiac Cell Analyses
08:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
已经开发并描述了用于从大鼠心脏分离不同心脏细胞类型的几种方案。在这里,描述了优化的方案,其允许从单一制剂分离高质量的主要心脏细胞类型(心肌细胞,内皮细胞和成纤维细胞),降低了实验成本。
Tags
Cardiomyocytes
Endothelial Cells
Fibroblasts
Cardiac Cells
Langendorff Perfusion
Collagenase Digestion
Cell Isolation
Cardiovascular Research
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