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Immunology and Infection
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慢性自身免疫性干眼鼠模型增加效应记忆T细胞在眼球组织
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
Please note that all translations are automatically generated.
Click here for the English version.
慢性自身免疫性干眼鼠模型增加效应记忆T细胞在眼球组织
DOI:
10.3791/55592-v
•
09:42 min
•
June 07, 2017
•
Aihua Hou
2
,
Tanima Bose
,
K. George Chandy
,
Louis Tong
2,4,5
1
Singapore Eye Research Insitute, A Member of SingHealth
,
2
DUKE-National University of Singapore Medical School
,
3
Lee Kong Chian School of Medicine
,
Nanyang Technological University
,
4
Singapore National Eye Center
,
5
Yong Loo Lin School of Medicine
,
National University of Singapore
Chapters
00:05
Title
01:18
Preparation of Lacrimal Gland Extract
02:32
Preparation of Emulsion
03:30
Immunization of Lewis Rats and Injection of Antigen Mixture into the Forniceal Subconjunctiva and Lacrimal Gland
04:53
Assessment of Dry Eye Features
07:19
Results
08:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
该报告描述了通过用大鼠泪腺提取物,卵清蛋白和完全弗氏佐剂的乳液免疫在Lewis大鼠中诱导慢性实验性自身免疫性干眼的方法,随后将泪腺提取物和卵清蛋白注射入穹隆结膜下和泪腺六周后。
Tags
Autoimmune Dry Eye
Rat Model
Lacrimal Gland
Effector Memory T Cells
Ocular Disease
Lymphocyte Trafficking
Sprague Dawley Rat
Lacrimal Gland Extract
Freund’s Adjuvant
Ovalbumin
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