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一个<em>体外</em> Caseum结合测定，预测药物在渗透结核病变
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Immunology and Infection
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JoVE Journal Immunology and Infection
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
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一个<em>体外</em> Caseum结合测定，预测药物在渗透结核病变

DOI:
10.3791/55559-v

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12:17 min

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May 08, 2017

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, , , , ,
1Public Health Research Institute Centre, New Jersey Medical School,Rutgers, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, NIAID,NIH

Chapters

  • 00:05Title
  • 01:02In Vitro Generation of Caseum Surrogate from THP-1 Cells
  • 05:23Rapid Equilibration Dialysis (RED) Assay
  • 08:02LC-MS Quantification and Data Analysis
  • 09:36Representative RED Assay Results
  • 11:27Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一种快速平衡透析（RED）方法，用于测量肺结核病变和腔内药物与病例的结合。该方案也与泡沫巨噬细胞衍生基质一起使用，这是一种有效的替代试剂。

Tags

In Vitro Caseum Binding AssayDrug PenetrationTuberculosis LesionsAntimicrobial PenetrationTHP-1 MonocytesIliac AcidPMACell CultureRPMI Medium

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