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Immunology and Infection
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哺乳动物细胞细菌挑战后应激颗粒形成的免疫荧光分析
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
Please note that all translations are automatically generated.
Click here for the English version.
哺乳动物细胞细菌挑战后应激颗粒形成的免疫荧光分析
DOI:
10.3791/55536-v
•
11:37 min
•
July 03, 2017
•
Pascale Vonaesch
,
Philippe J. Sansonetti
2
,
Pamela Schnupf
1
Unité de Pathogénie Microbienne Moléculaire, INSERM U786
,
Institut Pasteur
,
2
Microbiologie et Maladies Infectieuses
,
Collège de France
,
3
Laboratory of Intestinal Immunity, Institut Imagine-INSERM UMR 1163
,
Université Paris Descartes-Sorbonne Paris Cité
Chapters
00:05
Title
00:45
Bacteria:Host Cell Challenge
02:59
Stress Granule Formation Indication and Immunofluorescent Analysis
06:09
Fluorescence Imaging Analysis
08:47
Results: Representative Stress Granule Analysis
10:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了在用细菌和许多不同的应力攻击细胞后,哺乳动物细胞中应激颗粒形成的定性和定量分析的方法。该方案可用于研究广泛的宿主细菌相互作用中的细胞应激颗粒反应。
Tags
Immunofluorescence
Stress Granule
Bacterial Challenge
Mammalian Cells
Cellular Microbiology
Pathogen
Stress Granule Pathway
Image Analysis
S.flexneri M90T
Tryptic Soy Agar
Tryptic Soy Broth
Virulence Gene Expression
HeLa Cells
Infection Medium
Gentamicin
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