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该WinCF模式 - 粘液的廉价和易于处理的缩影细支气管堵塞，研究肺部感染的微生物
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Immunology and Infection
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JoVE Journal Immunology and Infection
The WinCF Model – An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections
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该WinCF模式 - 粘液的廉价和易于处理的缩影细支气管堵塞，研究肺部感染的微生物

DOI:
10.3791/55532-v

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06:57 min

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May 08, 2017

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, , , , , ,
1Skaggs School of Pharmacy and Pharmaceutical Sciences,University of California, San Diego, 2Department of Mechanical and Aerospace Engineering,University of California, San Diego

Chapters

  • 00:05Title
  • 00:38Preparing Medium and Artificial Sputum for Capillary Tube Loading
  • 02:29Filling the WinCF Capillary Tubes with Inoculated Artificial Sputum Medium
  • 04:12Incubation and Imaging the Extraction
  • 05:38Results: Microbiological Growth in the WinCF Capillary Tubes
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

粘液堵塞囊性纤维化的气道（CF）患者是微生物病原体茁壮成长的理想环境。手稿描述了用于研究CF肺微生物的环境中模仿其中它们引起的化学条件和疾病如何改变可驱动微生物动力学的新方法。

Tags

WinCF ModelArtificial Sputum MediumCystic Fibrosis LungAirway MicrobiologyBacterial PathogensMicrobial ResponsesMucus Plugged BronchioleLung InfectionsCapillary TubesHomogenized SputumIncubation TubesSterile Biohood

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