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Neuroscience
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斑马鱼<em>原位</em>脊髓制备用于脊髓感觉和运动神经元的电生理记录
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Zebrafish
In Situ
Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼<em>原位</em>脊髓制备用于脊髓感觉和运动神经元的电生理记录
DOI:
10.3791/55507-v
•
08:24 min
•
April 18, 2017
•
Rosa L. Moreno
,
Megan Josey
,
Angeles B. Ribera
2
1
Department of Physiology and Biophysics
,
University of Colorado Anschutz Medical Campus (UCAMC)
,
2
Neuroscience Graduate Program
,
University of Colorado Anschutz Medical Campus (UCAMC)
Chapters
00:05
Title
00:48
Preparation of Disseciton Materials
02:32
Dissection of Embryo for Patch-clamp Recordings from Spinal Neurons
07:00
Results: Excitability Properties of Bohon-Beard Neurons
07:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这手稿描述了从斑马鱼胚胎和幼虫的脊髓神经元的电生理记录的方法。制备维持神经元
在原地
,往往涉及到最小的清扫。这些方法允许对多种脊髓神经元的电生理学研究中,从通过早期幼体阶段的初始电兴奋采集。
Tags
Zebrafish
In Situ
Spinal Cord
Electrophysiology
Spinal Neurons
Sensory Neurons
Motor Neurons
Embryo
Larva
Rohon Beard Neurons
Silicone Elastomer
Dissection Chamber
Ringer’s Solution
Tricaine
Micropipette
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