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Journal
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Immunology and Infection
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IL-8瞬时转化的小鼠模型<em>体内</em>长期监测炎症反应
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Immunology and Infection
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JoVE Journal
Immunology and Infection
An IL-8 Transiently Transgenized Mouse Model for the
In Vivo
Long-term Monitoring of Inflammatory Responses
Please note that all translations are automatically generated.
Click here for the English version.
IL-8瞬时转化的小鼠模型<em>体内</em>长期监测炎症反应
DOI:
10.3791/55499-v
•
08:16 min
•
July 07, 2017
•
Gabriella Bergamini*
1
,
Fabio Stellari*
2
,
Angela Sandri*
3
,
Maria M. Lleo
,
Gaetano Donofrio
,
Francesca Ruscitti
2
,
Federico Boschi
,
Andrea Sbarbati
,
Gino Villetti
,
Paola Melotti
,
Claudio Sorio
1
Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory “D. Lissandrini”
,
University of Verona
,
2
Corporate Preclinical R&D
,
Chiesi Farmaceutici S.p.A.
,
3
Department of Diagnostic and Public Health, Microbiology Division
,
University of Verona
,
4
Dipartimento di Scienze Medico-Veterinarie
,
University of Parma
,
5
Department of Biomedical Biotechnological and Translational Sciences
,
University of Parma
,
6
Department of Computer Science
,
University of Verona
,
7
Department of Neurological, Biomedical and Movement Sciences
,
University of Verona
,
8
Cystic Fibrosis Regional Center (CFC)
,
AOUI Verona
Chapters
00:05
Title
01:07
In Vivo
Gene Delivery and Biomluminescent Imaging (BLI) and Photon Quantitification
04:24
Pro-inflammatory Stimuli Mouse Challenge
06:51
Results: Representative
P. aeruginosa
-induced Lung Inflammation Supernatent
In Vivo
Imaging in IL-8 Transiently Trangenic Mice
07:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里描述的方法允许通过非侵入性生物发光成像(BLI)在小鼠肺中IL-8启动子依赖性炎症激活的可视化。荧光素酶报告基因构建体的交付时间可以将相同的动物多次进行BLI多至两个月。
Tags
Keyword Extraction:IL-8
In Vivo
Lung Inflammation
Bacterial Culture Supernatant
Respiratory Infection
Gene Delivery
Bioluminescent Imaging
D-Luciferin
In Vivo Imaging System
Luminescent Imaging Mode
Photons
Article
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