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受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
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受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化

DOI:
10.3791/55445-v

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07:46 min

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April 03, 2017

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, , , , , , ,
1Department of Veterinary and Biomedical Sciences,The Pennsylvania State University, 2Microscopy and Cytometry Facility, The Huck Institutes of Life Sciences,Pennsylvania State University

Chapters

  • 00:05Title
  • 00:51Liver Tissue Isolation
  • 03:57Liver Cell Dissociation
  • 05:23Results: Representative Liver-isolated Macrophage Characterization
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

该协议允许研究员分离和表征组织驻留巨噬细胞在各种标志发炎组织提取从饮食诱发的代谢紊乱模型。

Tags

Macrophage IsolationPerfusionLiverObesity-related InflammationChronic InflammationImmunology

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