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蒸发减少培养条件的提高多细胞球体形成的微孔板重现
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Cancer Research
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JoVE Journal Cancer Research
Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates
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蒸发减少培养条件的提高多细胞球体形成的微孔板重现

DOI:
10.3791/55403-v

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11:24 min

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March 07, 2017

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, , , ,
1Institute of Molecular and Translational Medicine,Palacky University in Olomouc

Chapters

  • 00:05Title
  • 00:58Preparation of Agarose-coated Plates
  • 03:06Cell Culture and Multicellular Tumor Spheroid Formation
  • 06:17Medium Exchange Using a Robotic System
  • 07:14High-content Imaging of MCTS and Semi-automated Image Analysis
  • 08:04Analysis of Multicellular Spheroid Formation in the Presence of Anti-cancer Drugs
  • 10:16Conclusion

Summary

Automatic Translation

Automatic Translation

培养基从微量滴定板的凹凸损失影响均匀的多细胞肿瘤球体形成的再现性。改善培养条件，以减少显著介质损耗将提高球体形成的再现和基于球状体的测定的使用液体覆盖技术的结果。

Tags

3D Spheroid FormationMulticellular SpheroidsLiquid Overlay Technique384-well PlatesAgarose CoatingEvaporation ReductionReproducibilityScalabilityArthropod ScreeningEdge Effect

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