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Journal
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Immunology and Infection
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支气管肺泡灌洗小鼠肺分析炎症细胞浸润
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
Please note that all translations are automatically generated.
Click here for the English version.
支气管肺泡灌洗小鼠肺分析炎症细胞浸润
DOI:
10.3791/55398-v
•
07:03 min
•
May 04, 2017
•
Lien Van Hoecke
2
,
Emma Richelle Job
2
,
Xavier Saelens
2
,
Kenny Roose
2
1
UGent Center for Medical Biotechnology
,
VIB
,
2
Department of Biomedical Molecular Biology
,
Ghent University (UGent)
Chapters
00:05
Title
00:52
Broncoalveolar Lavage (BAL)
02:58
BAL Fluid Component Collection and Immunostaining for Flow Cytometry (FACS)
04:27
Results: Representative BAL Immune Cell Detection
06:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肺的健康状况的类型和存在于肺的细支气管的免疫细胞的数目反射。我们描述了支气管肺泡灌洗技术,它允许从小鼠的下呼吸道隔离和非粘附细胞和可溶性因子的研究。
Tags
Bronchoalveolar Lavage
Murine Lungs
Inflammatory Cell Infiltration
Respiratory Immunology
Lung Infection
Lung Inflammation
Innate Cellular Response
Humoral Response
BAL Fluid
Cell Harvesting
Ex Vivo Analysis
Disease Assessment
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