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Journal
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Immunology and Infection
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影像性流式细胞仪对人体免疫突触的定性和定量分析
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Immunology and Infection
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Immunology and Infection
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
影像性流式细胞仪对人体免疫突触的定性和定量分析
DOI:
10.3791/55345-v
•
08:35 min
•
January 07, 2019
•
Guido Wabnitz
,
Henning Kirchgessner
,
Yvonne Samstag
1
Institute of Immunology, Section Molecular Immunology
,
University of Heidelberg
Chapters
00:04
Title
00:38
Pan-leukocyte Preparation and Immune Synapse Induction and Staining
02:45
Flow Cytometric Image Acquisition
03:39
Data Analysis
05:54
Results: Representative CD3 and F-actin Immune Synapse Enrichment Analysis
07:22
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了一个完整的工作流程, 用于定性和定量分析免疫突触之间的原代人类 t 细胞和抗原呈现细胞。该方法基于成像流式细胞仪, 允许在相对较短的时间内采集和评估数千张细胞图像。
Tags
Immune Synapse
Leukocyte Communication
Imaging Flow Cytometry
T Cell Analysis
Staphylococcus Aureus Enterotoxin B (SEB)
Raji Cells
Pan-leukocytes
Paraformaldehyde Fixation
Saponin Permeabilization
Fluorophore-conjugated Antibodies
Flow Cytometry
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