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从小鼠在原代肝细胞培养物的炎性反应的诱导
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
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从小鼠在原代肝细胞培养物的炎性反应的诱导

DOI:
10.3791/55319-v

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08:32 min

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March 10, 2017

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, , , , ,
1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

Chapters

  • 00:05Title
  • 00:54Preparation for Perfusion and Liver Recovery
  • 03:23Isolation and Treatment of Cells
  • 05:23Generating cDNA from Isolated RNA by Reverse Transcription and Analysis of Cells by Quantitative PCR
  • 06:32Results: Imflammatory Response in Mouse Primary Hepatocyte Cultures
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

这里，我们表明酶促方法来原代肝细胞从成年小鼠隔离，我们描述了使用ELISA和实时PCR的炎症反应的量化。

Tags

HepatocytesInflammatory ResponseCytokinesCRPIL6Hepatic Acute Phase ResponseHepatocellular FunctionHepatic CytokinesChronic Kidney DiseaseHepatic MetabolomicsInflammationLiver PerfusionLiver DigestIVC CannulationLiver Dissection

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