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Biology
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定制室测定细胞迁移
JoVE Journal
Biology
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JoVE Journal
Biology
A Customizable Chamber for Measuring Cell Migration
Please note that all translations are automatically generated.
Click here for the English version.
定制室测定细胞迁移
DOI:
10.3791/55264-v
•
07:33 min
•
March 12, 2017
•
Aniqa N. Chowdhury
,
Huu Tri Vo
,
Sharon Olang
,
Elliott Mappus
,
Brian Peterson
,
Nora Hlavac
,
Tyler Harvey
,
Delphine Dean
1
Department of Bioengineering
,
Clemson University
Chapters
00:05
Title
00:39
Production of an Acrylic Mold of the Microchannel Chamber
01:37
Formation of a Microchannel Chamber
02:28
Sterilization of the Microchannel Chamber
03:15
Cell Seeding in the Microchannel Chamber
04:18
Formation and Chemotactic Factor Loading of an Agarose Block
05:19
Time-lapse Imaging of Cell Migration
05:43
Results: Time-lapse Cell Migration Tracking
06:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议细节来测量响应于也可用于确定药物的扩散速度出聚合物基质的化学引诱物细胞迁移可定制方法。
Tags
Cell Migration
Chemotactic
Chemorepulsive
Wound Healing
Micro-channel
CAD
Acrylic
Laser Cutting
PDMS
Elastomer
Vacuum
UV Sterilization
Cell Culture
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