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在缺血样条件下监测星形胶质细胞的反应性和增殖体外
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Neuroscience
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JoVE Journal Neuroscience
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
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在缺血样条件下监测星形胶质细胞的反应性和增殖体外

DOI:
10.3791/55108-v

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15:08 min

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October 21, 2017

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, , , ,
1Department of Neuroscience, School of Medicine,Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine,Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus,University of Puerto Rico

Chapters

  • 00:00Title
  • 01:01Isolation of Primary Astrocytes from Rat Cortices
  • 09:25Oxygen-Glucose Deprivation (OGD) of Primary Astrocytes
  • 13:40Representative Results
  • 14:08Conclusion

Summary

Automatic Translation

Automatic Translation

缺血性中风是一个复杂的事件, 其中星形胶质细胞对受氧葡萄糖剥夺 (OGD) 影响的脑区的具体贡献是很难研究的。本文介绍了一种获取孤立星形胶质细胞的方法, 并研究了其在 OGD 条件下的反应性和增殖。

Tags

AstrocyteAstrocyte ReactivityAstrocyte ProliferationIn VitroIschemic-like ConditionsOxygen Glucose Deprivation (OGD)GFAPPrimary Astrocytic Cell CulturePup Rat CorticesCerebral HemispheresCorticesMeningesDissection MicroscopeComplete DMEM Media

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