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Immunology and Infection
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で水平遺伝子伝達の研究のための方法論<em>黄色ブドウ球菌</em
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Methodology for the Study of Horizontal Gene Transfer in
Staphylococcus aureus
Please note that all translations are automatically generated.
Click here for the English version.
で水平遺伝子伝達の研究のための方法論<em>黄色ブドウ球菌</em
DOI:
10.3791/55087-v
•
10:39 min
•
March 10, 2017
•
Fabio Cafini*
1,2
,
Nguyen Thi Le Thuy*
3
,
Federico Román
,
José Prieto
,
Sarah Dubrac
7
,
Tarek Msadek
7
,
Kazuya Morikawa
1
Division of Biomedical Science, Faculty of Medicine
,
University of Tsukuba
,
2
Department of Basic Biomedical Science
,
Universidad Europea de Madrid
,
3
Human Biology Program, School of Integrative and Global Majors
,
University of Tsukuba
,
4
Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa
,
Instituto de Salud Carlos III
,
5
Division of Microbiology, Department of Medicine, School of Medicine
,
Universidad Complutense
,
6
Biology of Gram-Positive Pathogens, Department of Microbiology
,
Institut Pasteur, Paris, France
,
7
ERL3526
,
CNRS, Paris, France
Chapters
00:05
Title
00:51
Conjugation Using the Filter-mating Method
03:10
Phage Amplification on the Donor
04:43
Measuring the Phage Titer
06:01
Phage Transduction
07:54
Transformation Assay
09:41
Results: Successful Horizontal Gene Transfer
10:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは接合、形質導入、および
黄色ブドウ球菌
における自然形質転換の
in vitroでの
調査のための3つの異なるプロトコルを記述し
ます。
Tags
Horizontal Gene Transfer
Staphylococcus Aureus
Conjugation
Transduction
Natural Transformation
Antibiotic Resistance
CFR Gene
Donor
Recipient
Filter Membrane
Selective Agar
Colony PCR
Antibiogram
MIC
Disc Diffusion
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