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Journal
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Immunology and Infection
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葡聚糖增强小鼠和人原发性 NK 细胞的慢转导效率
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
Please note that all translations are automatically generated.
Click here for the English version.
葡聚糖增强小鼠和人原发性 NK 细胞的慢转导效率
DOI:
10.3791/55063-v
•
08:42 min
•
January 15, 2018
•
Arash Nanbakhsh
,
Brad Best
,
Matthew Riese
,
Sridhar Rao
,
Li Wang
,
Jeffrey Medin
,
Monica S. Thakar
,
Subramaniam Malarkannan
5,6,7
1
Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute
,
The Blood Center of Wisconsin
,
2
Vector Core Lab, Blood Research Institute
,
The Blood Center of Wisconsin
,
3
Laboratory of Lymphocyte Biology, Blood Research Institute
,
The Blood Center of Wisconsin
,
4
Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute
,
The Blood Center of Wisconsin
,
5
Department of Microbiology and Immunology
,
The Medical College of Wisconsin
,
6
Department of Pediatrics
,
The Medical College of Wisconsin
,
7
Department of Medicine
,
The Medical College of Wisconsin
Chapters
00:05
Title
00:44
Purification and Expansion of Murine Primary NK Cells
02:03
Purification and Expansion of Human Primary NK Cells
03:23
Transduction of Murine and Human Primary NK Cells with Lentivirus
06:25
Results: Lentiviral Transduction Efficiency Enhanced with Dextran
07:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
这项研究的目的是制定技术, 允许成功的基因转导在原自然杀手 (NK) 细胞。葡聚糖介导的人或小鼠原代 NK 细胞的慢转导可导致较高的基因表达效率。这种基因转导方法将极大地改善 NK 细胞的遗传操作。
Tags
Lentiviral Transduction
Primary NK Cells
Immunotherapy
Gene Transduction
Murine NK Cells
Human NK Cells
Cell Purification
Flow Cytometry
Viral Transduction
Dextran
Polybrene
Protamine Sulfate
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