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Journal
/
Immunology and Infection
/
在
从幼稚的CD4
+
T细胞
的
人CD4
+
FOXP3
+
诱导调节性T细胞(iTregs的)使用含有TGF-β-协议的
体外
分化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
In Vitro
Differentiation of Human CD4
+
FOXP3
+
Induced Regulatory T Cells (iTregs) from Naïve CD4
+
T Cells Using a TGF-β-containing Protocol
Please note that all translations are automatically generated.
Click here for the English version.
在
从幼稚的CD4
+
T细胞
的
人CD4
+
FOXP3
+
诱导调节性T细胞(iTregs的)使用含有TGF-β-协议的
体外
分化
DOI:
10.3791/55015-v
•
08:20 min
•
December 30, 2016
•
Angelika Schmidt
,
Szabolcs Éliás*
1
,
Rubin N. Joshi*
1
,
Jesper Tegnér
1
Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna
,
Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
Chapters
00:05
Title
01:03
Peripheral Blood T Cell Isolation
03:43
Treg Induction, Plating and Monitoring, and Staining
05:52
Results: Representative CD4
+
FOXP3
+
iTreg Analyses
07:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述从幼稚CD4
+
T细胞
在体外
可重复产生和人为调节性T细胞(iTregs的)的表型。不同的协议FOXP3诱导允许与各自的协议获取特定表型iTreg的研究。
Tags
In Vitro
Differentiation
Human
CD4+FOXP3+
Induced Regulatory T Cells
ITregs
Naive CD4+ T Cells
TGF-β
Phenotype
T Cell Immunology
PBMC
Magnetic Bead Isolation
Biotin Antibody
Anti-biotin Microbeads
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