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1Singapore Immunology Network (SIgN),Agency for Science, Technology and Research (A*STAR), Biopolis, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 3Lee Kong Chian School of Medicine,Nanyang Technological University, 4Centenary Institute for Cancer Medicine and Cell Biology, 5Discipline of Dermatology,University of Sydney, 6Department of Dermatology,Royal Prince Alfred Hospital, 7LSI Immunology Programme,National University of Singapore, 8School of Biological Sciences,Nanyang Technological University
Chapters
- 00:05Title
- 00:53Mouse Anesthesia and Depilation
- 01:35Induction of Ischemia and Reperfusion Injury
- 02:31Placement of the Ear on the Imaging Platform
- 04:33Results: Neutrophil Recruitment in Ischemia-reperfusion Injury
- 05:20Conclusion
Questo protocollo descrive l'induzione di un modello di ischemia-riperfusione (IR) sulla pelle orecchio mouse utilizzando magnete di bloccaggio. Utilizzando un modello di imaging intravitale custom-built, studiamo in vivo risposte infiammatorie post-riperfusione. La logica alla base dello sviluppo di questa tecnica è quello di estendere la comprensione di come leucociti rispondono al danno IR pelle.
