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对于神经化学标记用biocytin填充和加工部分的免疫染色
JoVE Journal
Neuroscience
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Neuroscience
Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers
Please note that all translations are automatically generated.
Click here for the English version.
对于神经化学标记用biocytin填充和加工部分的免疫染色
DOI:
10.3791/54880-v
•
08:54 min
•
December 31, 2016
•
Bogumila Swietek
,
Akshay Gupta
,
Archana Proddutur
,
Vijayalakshmi Santhakumar
1
Graduate School of Biomedical Sciences
,
Rutgers New Jersey Medical School
,
2
Department of Pharmacology, Physiology and Neuroscience
,
Rutgers New Jersey Medical School
Chapters
00:05
Title
01:19
Biocytin-filling during Electrophysiology
03:31
Staining of the First Primary Antibody and Biocytin
04:58
Staining of the Second Primary Antibody
06:32
Results: Successful CCK Staining 1 Week Following the Recovery of Biocytin Staining and CB1R Labeling
07:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提出了在使用生物胞素填充和随后的免疫组织化学后处理电生理记录修补神经元的形态回收的方法。我们表明,该染色和盖片厚生物胞素填充部分可以与第二初级抗体天或数月后进行复染。
Tags
Biocytin
Immunostaining
Neurochemical Markers
Neuronal Structure
Neuronal Function
Brain Networks
Morphological Recovery
Whole-cell Recording
Electrophysiology
Cell Patching
Cell Filling
Capacitive Transients
Series Resistance
Voltage Clamp
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