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Journal
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Immunology and Infection
/
乙型肝炎病毒记者系统的开发监控复制周期的早期阶段
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Please note that all translations are automatically generated.
Click here for the English version.
乙型肝炎病毒记者系统的开发监控复制周期的早期阶段
DOI:
10.3791/54849-v
•
09:35 min
•
February 01, 2017
•
Hironori Nishitsuji
,
Hiromi Yamamoto
,
Ritsuko Shiina
,
Keisuke Harada
,
Saneyuki Ujino
,
Kunitada Shimotohno
1
Research Center for Hepatitis and Immunology
,
National Center for Global Health and Medicine
,
2
Central Pharmaceutical Research Institute
,
Japan Tobacco Inc.
Chapters
00:05
Title
00:42
Production of Recombinant Hepatitis B Virus Encoding the Reporter Protein
03:33
Infection of Recombinant Hepatitis B Virus
05:09
Analysis of Hepatitis B Virus Infection
05:56
Results: HBV Reporter Activity in HepG2 Cells Infected with Recombinant Hepatitis B Virus
07:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里,我们描述了新开发的乙型肝炎病毒(HBV)报告系统来监视HBV生命周期的早期阶段。
该体外
系统的简化将在使用高通量策略抗HBV剂的筛选帮助。
Tags
Hepatitis B Virus
HBV
Reporter System
HepG2 Cells
Transfection
Recombinant HBV
Cell Culture
Virus Replication
Virus Purification
NTCP
Infection
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