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Journal
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Immunology and Infection
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在动物标本呼吸道病原体的高通量检测通过纳米PCR
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Please note that all translations are automatically generated.
Click here for the English version.
在动物标本呼吸道病原体的高通量检测通过纳米PCR
DOI:
10.3791/54781-v
•
11:00 min
•
November 28, 2016
•
Laura B. Goodman
,
Renee R. Anderson
,
Marcia Slater
,
Elen Ortenberg
,
Randall W. Renshaw
,
Brittany D. Chilson
,
Melissa A. Laverack
,
John S. Beeby
,
Edward J. Dubovi
,
Amy L. Glaser
1
Population Medicine and Diagnostic Sciences
,
Cornell University Animal Health Diagnostic Center
,
2
Thermo Fisher Scientific Inc.
Chapters
00:05
Title
01:00
Reverse-transcription/Pre-amplification (Preamp)
03:47
Liquid Handler Transfer
07:58
Result Analysis
09:15
Results: Representative Results from an RNA Assay, Influenza Virus A, and a DNA Assay, Equine Herpesvirus Type 1
10:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
的DNA和RNA基于病原体由纳米级的PCR的高通量的检测是使用综合征犬和马呼吸PCR面板说明。
Tags
Keyword Extraction:High-throughput Detection
Respiratory Pathogens
Animal Specimens
Nanoscale PCR
Real-time PCR
DNA
RNA
Pathogens
OneHealth Surveillance
Infectious Diseases
Clinical Specimens
Respiratory Pathogens
Horses
Dogs
Lysis Buffer
Reverse Transcription
Pre-amplification
Preamp
96-well Plate
Thermocycler
CDNA Production
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