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Immunology and Infection
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一种用于丝状真菌菌落细菌生物膜的定性多尺度分析的新方法利用共聚焦和电子显微镜
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
一种用于丝状真菌菌落细菌生物膜的定性多尺度分析的新方法利用共聚焦和电子显微镜
DOI:
10.3791/54771-v
•
09:45 min
•
January 25, 2017
•
Cora Miquel Guennoc
,
Christophe Rose
,
Frédéric Guinnet
,
Igor Miquel
,
Jessy Labbé
,
Aurélie Deveau
1
Interactions Arbres – Microorganismes, UMR1136
,
INRA Université de Lorraine
,
2
Ecologie et Ecophysiologie Forestières – PTEF, UMR 1137
,
INRA Université de Lorraine
,
3
Biosciences Division
,
Oak Ridge National Laboratory
Chapters
00:05
Title
00:50
Preparation of Bacterial Fungal Cultures
02:11
Preparation of
In Vitro
Biofilm of Bacteria on the Fungal Colony
03:41
Sample Preparation for Confocal Microscopy Analysis of Biofilm Formation
05:35
Confocal and Electron Microscopic Analysis
07:07
Results: Meso- and Micro-scale Analysis of
P. fluorescens
BBc6 on
L. bicolor
08:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个协议描述成长和定性通过共聚焦和电镜分析关于真菌菌丝细菌生物膜的新方法。
Tags
Bacterial Biofilm
Filamentous Fungal Colonies
Confocal Microscopy
Electron Microscopy
Multi-scale Analysis
Cellophane Membrane
EDTA
Fungal Pre-culture
P. Fluorescens
Potassium Phosphate Buffer
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