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一种用于丝状真菌菌落细菌生物膜的定性多尺度分析的新方法利用共聚焦和电子显微镜
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
DOI:

09:45 min

January 25, 2017

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Chapters

  • 00:05Title
  • 00:50Preparation of Bacterial Fungal Cultures
  • 02:11Preparation of In Vitro Biofilm of Bacteria on the Fungal Colony
  • 03:41Sample Preparation for Confocal Microscopy Analysis of Biofilm Formation
  • 05:35Confocal and Electron Microscopic Analysis
  • 07:07Results: Meso- and Micro-scale Analysis of P. fluorescens BBc6 on L. bicolor
  • 08:29Conclusion

Summary

Automatic Translation

这个协议描述成长和定性通过共聚焦和电镜分析关于真菌菌丝细菌生物膜的新方法。

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