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由荧光相关光谱测量的一次淋巴细胞的细胞膜分子扩散
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
DOI:

12:06 min

February 01, 2017

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Chapters

  • 00:05Title
  • 01:07Starting the FCS System
  • 02:26Pinhole Adjustment
  • 04:41Measure the Transit Time of the Free Fluorophore
  • 05:50Measure the Transit Time of Free Antibody
  • 06:51Cell Measurements
  • 09:47Results: Assessing the Autocorrelation Curve
  • 11:04Conclusion

Summary

Automatic Translation

A method to measure protein diffusion in membranes of primary immune cells using fluorescence correlation spectroscopy (FCS) is described. In this paper, the use of antibodies for fluorescent labeling is illustrated.

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