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重组离子通道的相对细胞表面表达的总用流式细胞仪测定
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Biology
Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
Please note that all translations are automatically generated.
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重组离子通道的相对细胞表面表达的总用流式细胞仪测定
DOI:
10.3791/54732-v
•
11:32 min
•
September 28, 2016
•
Benoîte Bourdin
,
Emilie Segura
,
Marie-Philippe Tétreault
,
Sylvie Lesage
,
Lucie Parent
1
Département de Physiologie Moléculaire et Intégrative
,
Montreal Heart Institute Research Centre
,
2
Département de Microbiologie, Infectiologie, Immunologie
,
Centre de recherche de l’Hôpital Maisonneuve-Rosemont
Chapters
00:05
Title
01:08
Liposome Mediated Transient Transfection
02:35
Preparing Cell Samples for Staining
04:51
Extracellular Staining of Cell Samples
06:10
Intracellular Staining of Cell Samples
07:51
Data Analysis
08:55
Results: Simultaneaous Mutations of N-glycosylation Sites Disrupted Cell Surface Expression of CaVα2δ1
10:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
继承心律失常通常是由改变一种或多种离子通道的表面上输送的突变引起的。在这里,我们适应流式细胞测定法,以提供在TSA-201细胞中表达的重组的离子通道的相对总和细胞表面蛋白表达的定量。
Tags
Flow Cytometry
Recombinant Ion Channels
Cell Surface Expression
Membrane Proteins
Fluorescent Assay
Cardiac Arrhythmias
MCherry
Hemagglutinin Epitope
TSA201 Cells
Transfection
Trypsin-EDTA
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