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去除<em>果蝇</em从幼虫鱼片的感官神经元和表皮细胞的免疫荧光分析>肌肉组织
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
DOI:

06:41 min

November 02, 2016

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Chapters

  • 00:05Title
  • 00:41Preparation and Dissection of Larvae
  • 02:34Fixation and Mounting of Larval Fillets
  • 04:10Results: Muscle Removal Enables Enhanced Imaging of Da Neurons and the Epidermis
  • 06:02Conclusion

Summary

Automatic Translation

使用的果蝇幼虫树突分支(DA)神经元的神经元形态的研究通过免疫荧光神经细胞和表皮蛋白质的原位可视化中受益。我们描述通过从幼虫体壁除去肌肉组织提高多巴胺神经元和包围的表皮细胞的免疫荧光分析的过程。

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